Novel Real-Time PCR Assay for Simultaneous Detection and Differentiation of Clostridium chauvoei and Clostridium septicum in Clostridial Myonecrosis
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چکیده
منابع مشابه
Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis.
A real-time PCR assay based on the 16S rRNA gene sequence was designed for differentiation of blackleg-causing Clostridium chauvoei and Clostridium septicum, a phylogenetically closely related bacterium responsible for malignant edema. In order to exclude false-negative results, an internal amplification control was included in the assay. A set of three probes, one specific for C. chauvoei, one...
متن کاملComplexity of toxins from Clostridium septicum and Clostridium chauvoei.
Production of toxins by Clostridium septicum and Clostridium chauvoei has been the subject of many conflicting reports. Roux and Chamberland (1887) first noted that 3 to 4 day filtrates were antigenic; conferring on guinea pigs an immunity against infection with viable organisms. Production of a lethal toxin by C. septicum was however, demonstrated by Leclainche (1898) and Leclainche and Morel ...
متن کاملClostridium septicum myonecrosis in congenital neutropenia.
Severe congenital neutropenia (SCN) and Clostridium septicum myonecrosis is an uncommon and life-threatening association requiring urgent combined aggressive medical and surgical management. We report 2 cases of SCN (1 with known Kostmann's syndrome and 1 not known at presentation to have a congenital neutropenic disorder but subsequently received a diagnosis of cyclic neutropenia) who presente...
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Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملmultiplex real-time pcr assay for detection and differentiation of bordetella pertussis and bordetella parapertussis.
background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01975-09